重组人绒毛膜促性腺激素β-亚基对C57BL小鼠免疫应答反应及抗血清特征的研究

The Immunological Response of Recombinant β-subunit of Human Chorionic Gonadotropin in C57 Black Mouse and the Properties of it's Antisera

采用福氏性剂，对C57BL小鼠进行重组hCGβ和天然hCGβ免疫应签反应的比较研究，结果表明：两者引发的抗血清性质相似，与hCG有较高的亲和力（K（γβ）≈5．86×108／mol／L，Kaβ≈8．18×108／mol／L）；抗血清能有效地抑制（125）I－hCG与睾丸受体的结合，结果提示重组hCGβ抗血清与天然hCGβ抗血清相似，具有中和hCG的生物活性能力，重组hCGβ可作为免疫避孕疫苗的抗原。

By using Freund's adjuvant the immunological response of C57 black mouse to recombinant hCGS and natural hCGβ was comparatively investigated. The properties and merits of the antibodies elicited by recombinant hCGβ were similar in comparison with them induced by natural hCGβ.The antisera had high affinity for binding with hCG (Ka(γβ≈5. 86×108/mol/L,Kaβ=8. 18 ×108/ mol/L),and were also found to be effective in preventing the binding of (125)I-hCG to receptors in rat testes. The sera were able to neutralize the biological activities of hCG. These investigations show that recombinant hCGβ could be used as immunogen for contraceptive vaccine.