银屑病患者骨髓CD34^＋细胞定向分化的T细胞对角质形成细胞增殖的影响

Influence of T cells differentiated from bone marrow-derived CD34^+ cells of psoriatic patients on keratinocyte proliferation in vitro

目的:探讨家族史阳性的银屑病患者骨髓CD34+细胞体外定向分化的T细胞对表皮角质形成细胞(KC)增殖调控蛋白表达的影响。方法:将银屑病患者骨髓CD34+细胞体外定向分化的T细胞经链球菌超抗原活化后与KC共培养,分别采用免疫组化法及ELISA法检测KCC-myc、bcl-xL、p53及Ki67蛋白表达及培养上清白介素(IL)-8、干扰素(IFN)-γ水平。结果:①受银屑病患者CD34+细胞定向分化T细胞作用的KCC-myc及Ki67蛋白表达与自然增殖组及正常人CD34+细胞定向分化的T细胞作用组相比显著增强(P<0.05),而bcl-xL及p53蛋白表达3组间的差异无统计学意义(P>0.05);②受正常人CD34+细胞定向分化T细胞作用的KCC-myc、bcl-xL、p53及Ki67蛋白表达与自然增殖组比较差异亦无统计学意义(P>0.05);③银屑病CD34+细胞定向分化的T细胞作用组培养上清IL-8及IFN-γ水平显著高于正常对照组(P<0.01)。结论:银屑病患者骨髓造血细胞定向分化的T细胞可影响KC增殖状态,显示类似银屑病外周血T细胞的活性特点。

Objective： To explore the effect of T cells differentiated from bone marrow-derived CD34^＋ cells of psoriatic patients on keratinocytes. Methods： Keratinocytes （KCs） from foreskin were cultured with or without T cells, differentiated from bone marrow-derived CD34^＋ hematopoietic cells from psoriatic patients with family history or normal controls and activated by streptococcal superantigen （SAg）. C-myc, bcl-xL, p53 and Ki67 proteins on KCs were determined by immunohistochemistry and IL-8 and IFN-γ levels in the culture supematant were detected by enzyme linked immunosorbent assay （ELISA）. Results： C-myc, bcl-xL, p53 and Ki67 proteins were expressed at low density and proportion in KCs cultured with the T cells from normal controls and did not show significant difference with those without any T cell stimulation. However, C-myc and I（367 protein expression was greatly increased after KCs were cultured with T cells differentiated from bone marrow CD34^＋ cells of psoriatic patients with higher IL-8 and IFN-γ levels in supematant. Conclusions; T cells derived from hematopoietic cells of psoriatic patients with family history are abnormal in their functions similar to psoriatic circulating T cells since they can influence proliferation of, keratinocytes probably by secreting cytokines.