国产ELISA试剂盒应用于兴奋剂控制中的hCG检测

Determination of hCG in doping control by domestic ELISA kits

本文介绍用国产酶免定量药盒测定血、尿样中ｈＣＧ含量的一种ＥＬＩＳＡ方法。该方法特异性好、经济可靠而且简便快捷，适用于运动员尿样的兴奋剂检测。文中还报道了该方法应用于测定两例志愿者注射ｈＣＧ后的血、尿样及国际奥委会医学委员会１９９４年水平考试和１９９５年资格复试ｈＣＧ阳性尿样的结果，并讨论了测得值与其它试剂盒测定结果的差异及原因。

A domestic quantiative ELISA kit was used in the determination of hCG in 2 volunteers who had been administered 2000 IU hCG intramuscularly.HCG was detectable in serum and urine within 4 to 5 days' period after administration.The kit was also used in the I.O.C. Proficiency Test (1994) and the Reaccreditation Test (1995) for accredited doping control laboratories and the results were given and discussed .