血浆中盐酸氟桂嗪的荧光分光光度测定法

Determination of Flunarizine Hydrochloride in Human Plasma by Fluorometry

建立了血浆样品中盐酸氟桂嗪的荧光分光光度测定法。血浆样品经碱化用正己烷提取，水浴挥干正己烷后，用0．1mol／LHCI定容，在激发波长260um、发射波长310um处测定荧光强度。最低检出浓度10ng／ml。线性范围2．0—30．0μg／ml（r=0．9907）。平均口收率为100．08％，RSD为5．03％（n＝9），日内、日间精密度分别为1．63％～3．28％和1．94％～5．95％。方法简便、快速、准确。

Afluorometricmethodwasestablishedforthedeterminationofflunarizinehydrochlorideinhumanplasma.Thedrugwasextractedfromakalizedplasmaintonhexane.Oxganicextractantwasdriedunderbathwater.Theresiduewasdissolvedin0.1mol/LHCl.Fluorescencewasmeasuredundertheconditionswhichexcitationandemissionwavelengthswereset260nmand310um.Thelowestdetectiveconcentrationofflunarizinehydrochloridewas10ng/ml.Linearcalibrationcurveforflunarizinehydrochloridewasmeasuredwitharangeof2.0to30.0ug/ml(withthecorrelationcoeffidentof0.9907).Theaveragerecoyerywas100.08N,theRSDwas5.03%(n=9).TheRSDofwithin－dayandbetween－daywere1.63%to3.28%and1.94%to5.95%,respectively.Themethodissimple,fastandaccureate.Keyowrds:flunarizine hydrochloride,fluorometry,plasma concentration