强的松联合依那普利对实验性IgA肾病小鼠血尿、蛋白尿、肾功能及肾脏病理的影响

The influences of prednisone combining enalapril on the clinic outcomes and the renal pathological parameters of IgAN mice

目的探讨强的松联合依那普利对IgA肾病(IgAN)小鼠血尿、蛋白尿、肾功能及肾脏病理的影响是否优于单用强的松或单用依那普利。方法用"口服牛血清清蛋白加葡萄球菌肠毒素B尾静脉注射"法复制小鼠IgAN模型。设正常组、模型组、强的松组、依那普利组、强的松联合依那普利组。实验结束时,比较各组小鼠血尿、尿蛋白定量、血尿素氮、肌酐、肾组织形态学及免疫荧光半定量的差异。结果模型组小鼠24h尿蛋白定量、血尿定性、血尿素氮及肌酐、肾脏系膜区IgA荧光强度、肾脏系膜基质表达均明显高于正常组(P<0.05);强的松组、依那普利组和强的松联合依那普利治疗组上述指标分别低于模型组,差异均有统计学意义(P<0.05);强的松联合依那普利治疗组上述指标分别低于单用强的松组或单用依那普利组(P<0.05);强的松组上述指标低于依那普利组(P<0.05)。结论在减少IgAN小鼠的血尿和蛋白尿、改善小鼠系膜基质的沉积,稳定小鼠的肾功能方面,单用强的松疗效优于单用依那普利,合用强的松和依那普利疗效优于单用强的松或单用依那普利。

Objective To study the different influences between the signal use of prednisone or enalapril and the combining of prednisone and enalapril on the clinic outcomes and the renal pathological parameters of IgAN mice. Methods The IgAN model was built by the method of oral intake of bovine serum albumin （ BSA ） together with the injection of staphylococcus enterotoxin B （ SEB ） through caudal vein. The urine protein quantitative, hematuria qualitative and renal function were tested, The pathological parameters：area of mesangial region, area of renal glomruli and the ratio of the former to the latter, IgA immunofiuorescence in mesangial region were tested. Results The results of urine protein quantitative examination, hematuria qualitative and renal function test of mice in model group were respectively higher than those of normal group （ P 〈 0.05 ）. The whole parameters in the prednisone combining enapril group were respectively lower than those in the prednisone group and enalapril group（ P 〈 0.05 ）, while the whole parameters in the prednisone group were lower than those in enalapril group（ P 〈 0.05 ） . Conclusion Prednisone and enalapril all can alleviate the hematuria, proteinuria, pathological change, stabilize the renal function of the IgA nephropathy mice, but prednisone combining enalapril had better effects than single use of prednisone or enalapril.