琼脂糖等电聚焦法分离碱性磷酸酶同工酶在骨质疏松诊断中的应用

The application of alkaline phosphatase isoenzyme in the diagnosis of osteporosis by agarose isoelectricfocusing

目的探讨碱性磷酸酶(ALP)同工酶在骨质疏松诊断中的应用。方法采用神经氨酸苷酶处理标本,用琼脂糖等电聚焦法(IEF)分离测定50例健康体检者和55例骨质疏松患者血清中的ALP同工酶。结果经神经氨酸苷酶处理后,用琼脂糖IEF能分离肝和骨ALP。发现骨质疏松患者中31例有一等电点(pI)为6.8的区带,阳性率为56.4%。结论改良的琼脂糖IEF法能分离ALP同工酶,pI6.8的区带对骨质疏松的诊断具有一定的价值。

Objective To discussion alkaline phosphatase（ALP）isoenzyme in the diagnosis of osteporosis. Methods Separated by neuraminidase, ALP isoenzyme in the serum of 50 healthy people and 55 osteporosis were analyzed by agarpse isoelectricfocusing （IEF） . Results Liver and bone ALP can be separated by agarose IEF after using neuraminidase. There was a pI 6,8 band which did not exit in healthy group but in osteporosis group, The positive ratio was 56.4% . Conclusion ALP isoenzyme can be separated by improved agarose IEF,There was a relationship between pI 6.8 band and osteporosis,