静磁场对鼠成骨细胞增殖和胞内钙离子浓度的影响

Effect of static magnetic field on intracellular calcium concentration of osteoblast cell

目的:观察静磁场对大鼠成骨细胞的增殖和在一定磁感应强度静磁场作用下胞内钙离子浓度的变化,以期探讨静磁场在促成骨细胞增殖中可能的作用机制。方法:体外培养Wistar大鼠颅骨成骨细胞,置于0、0.026、0.044、0.090T的磁感应强度下,利用MTT法检测24、48、72h的增殖率。利用荧光双波长分光光度计测定0.044T磁感应强度的静磁场作用下钙离子在成骨细胞内的浓度变化。采用SPSS11.0统计软件对结果进行单因素方差分析(ANOVA)、t检验。结果:磁感应强度为0.044T时,成骨细胞在48h内出现明显增殖(P<0.01)。静磁场作用72h,0.044T组和0.090T组的增殖率与0T组之间存在显著性差异(P<0.01)。成骨细胞在0.044T静磁场作用5、10min时,胞内钙离子浓度与对照组比较无显著性差异,作用15min时,与对照组之间存在显著性差异(P<0.01)。结论:静磁场能促进成骨细胞的增殖,并可能存在一定的强度和时间依赖性。一定磁感应强度的静磁场能使成骨细胞内钙离子浓度升高,并存在一定的时间依赖性。

Objective ：To observe the proliferation and the free intracellular calcium ion concentration of osteoblast under the effect of static magnetic field and discuss the possible mechanism of the static magnetic field on promoted the proliferative function. Methods： The MTT assay was used to measure the rate of the proliferation on different magnetic field intensity （0, 0. 026, 0. 044, 0. 090 T） and action time （ 24, 48, 72 h）. The fluorescence double wavelength spectrophotometer was used to measure the concentration of intracellular calcium ion. The data obtained were analyzed for ANOVA and t-test using SPSS 11.0 software package. Results ： Osteoblasts in group 0. 044 T appeared significant proliferation within 48 h（ P 〈 0.01 ）. When the static magnetic field effected on the cell at 72 h, the group 0. 044 T and group 0. 090 T were rising significantly comparing with group 0 T（ P 〈 0.01 ）. The [ Ca2＋ ] i of osteoblast in the group 15 rain was increased significantly under the static magnetic field of 0. 044 T. Conclusion：The static magnetic field can accelerate the proliferation of the cultured osteoblast in the intensity and time dependent way. Certain intensity of static magnetic field can increase the concentration of the intracellular calcium ion, and may be dependent on time.