滞留时间法评价羧甲基纤维素钠水凝胶经皮释药特性

Evaluation on the permeation of ibuprofen through sodium carboxymethyl cellulose gel by lag time method

【目的】研究不同浓度的羧甲基纤维素钠对凝胶剂中布洛芬经皮渗透特性的影响。【方法】采用改良的Franz扩散池,以离体鼠皮为透皮屏障,用紫外分光光度法测定接受液中布洛芬的含量。考察不同浓度凝胶基质中布洛芬在不同时间的累积透过量,采用滞留时间法评价羧甲基纤维素钠水凝胶的经皮释药特性。【结果】1.25%和2.5%甲基纤维素的布洛芬凝胶剂的外观较好,3种基质凝胶剂中布洛芬的累积透过量无显著性差异。3种布洛芬凝胶剂的稳态流量、渗透系数没有差异,表观扩散系数、表观分配系数以及滞留时间略有不同。【结论】选用的3个浓度基质布洛芬的经皮释药特性没有显著差异,临床应用该基质应考虑凝胶剂的外观和涂展性。

[ Objective] To study the effeets of different concentration of sodium carboxymethyl cellulose gel in permeating ibuprofen through skin in vitro . [Methods] Using improved Franz type diffusion cell and excised rat skin in vitro as transdermal barrier, the concentration of ibuprofen in samples was determined by UV method. The cumulative permeation quantities of ibuprofen in gel were observed at0.5, 1, 1.5, 2, 3, 4, 5, 6, 8, 10, 24, 48 and72h, respectively. The characteristics of percutaneous permeation for ibuprofen in sodium carboxymethyl cellulose hydrogel was evaluafed by was evafuafed lag time method. [ Resuits] The appearance of ibuprofen gel was very good with sodium carboxymethyl cellulose at the concentration of 1.25% and 2.5%. The amount of wausdermal absorption of ibuprofen in three different concentration gels were not differenct, significanOy. And the the steady-state flux （Jss） was not differenct, too. Lag time （Tlag）, the diffusion coefficent （Dapp）, and skin/donor phase partition coefficient （Kapp） had a little o difference. [ Conclusions] Percutaneous ibuprofen fiu-ough sodium carboxymethyl cellulose hydrogel in different concentration was not differenct, and we must think of the appearance and daub capability of gel.