多发性骨髓瘤细胞株RPMI8226上清液抑制成骨细胞早期分化

Suppression effect of supernatant from Mutiple myeloma cells RPMI8226 on the early stage of osteoblast differentiation

目的:探讨多发性骨髓瘤细胞上清液在体外抑制成骨祖细胞早期分化的现象。方法:以多发性骨髓瘤细胞株RPMI8226上清加入到成骨祖细胞MC3T3-E1rBMP2诱导分化体系中培养6d后,计数碱性磷酸酶染色结节并以RT-PCR方法测定碱性磷酸酶和骨钙素mRNA水平。结果:MC3T3-E1诱导组和30%的骨髓瘤上清干预组的染色结节计数分别为(20.33±5.16)/低倍视野和(13.66±2.80)/低倍视野(P<0.05)。此外,30%上清干预组的碱性磷酸酶mRNA水平低于诱导组(P<0.05),而骨钙素mRNA水平差异无显著性(P>0.05)。结论:多发性骨髓瘤细胞RPMI8226上清液能够抑制rBMP2诱导的成骨祖细胞MC3T3-E1早期分化过程。

Objective： To investigate the effect of supernatant from Mutiple myeloma cells RPMI8226 on the early stage of osteoblast differentiation in vitro. Methods： The supernatant from Mutiple myeloma cells RPMI8226 was added into the system of the osteoblast differentiation which induced by rBMP2. After 6 days, osteoblast was collected to identify ALP staining nodule counting and mRNA of ALP and OC with RTPCR. Results： The ALP staining nodule counting was （13.66 ± 2.80） /L-visualis in supernatant interven- tion group and it was, （20.33 ± 5.16） /L-visualis in control group.The level of ALP mRNA was lower in supernatant intervention group than that of control group（P〈0.05）. But the level of 0cmRNA in both group was indiscrimination（P〉0.05）. Conclusion： The supernatant from Mutiple myeloma cells RPMI8226 inhibits the the early stage of osteoblast MC3T3-E1 differentiation which induced by rBMP2.