食管鳞癌淋巴结转移的蛋白质组分析

Proteomic analysis of esophageal squamous cell carcinoma cells between carcinoma tissue and metastatic lymph nodes

分析食管鳞癌转移淋巴结中鳞癌细胞与食管鳞癌组织中鳞癌细胞的蛋白质的表达差异,获取鉴别两者的分子标志物。用激光捕获显微切割技术分离出食管鳞癌转移淋巴结和食管鳞癌组织中较纯的鳞癌细胞,运用双向电泳和质谱的方法检测两者表达的差异蛋白,并用免疫印迹技术对差异候选蛋白进行分析、验证。发现29个差异蛋白点,通过质谱鉴定出6种有意义的蛋白,转移淋巴结中的鳞癌细胞中如peroxiredoxin 1等5种蛋白表达明显增高,1种蛋白MTCBP-1表达下调。因此激光捕获显微切割可以有效地解决组织异质性的问题;食管鳞癌组织中的鳞癌细胞与转移淋巴结中的鳞癌细胞的2-DE蛋白质图谱具有明显的差异表达,提示食管鳞癌的淋巴结转移过程的发生是多种蛋白质功能共同作用的结果,从而造成肿瘤细胞迁移性和侵袭性的进一步增强。

To explore the biomarkers for differential diagnosis of esophageal squamous cell carcinoma （ESCC） cells between carcinoma tissue and metastatic lymph nodes, laser capture microdissection was used to separate them. For them, different proteome were observed using a 2-D polyacrylamide gel electrophoresis technique and electrospray ionisation tandem mass spectrometry. 29 protein spots were discovered, and 6 significant different proteins were identified. Therein, 5 proteins as peroxiredoxin 1 in gelsolin family-capping proteins increased expression. By contrast, 1 protein as MTCBP-1 proteins in the esophageal squamous cell of metastatic lymph nodes decreased expression. The results show that laser capture microdissection is a key technique in proteomic studies and can deal effectively with the problem of irrelevant tissues. Various proteins may be involved in the metastasis of esophageal squamous cell carcinoma.