摘要
从松树叶面分离到1批拮抗细菌,经过室内外活体接种防病试验,最终筛选出1株对松枯梢病表现出良好防病潜能的生防菌株NA。常规鉴定和Biolog鉴定结果表明其为枯草芽孢杆菌种(Bacillus subtilis)。克隆了NA的16 S rDNA,通过测定和比较16 S rDNA的部分序列,对NA进行了分子鉴定,进一步验证了种的鉴定结果的正确性。
A lot of antagonism bacteria isolations were obtained from leaves of pines. Through test on the seedling of pines indoor and outdoor,the antagonistic strain NA which had strong biocontrol effect against Sphaeropsis sapinea was obtained. The result of regular identification and biolog dentification demonsrated that NA was Bacillus subtilis was obtained. The research cloned 16 S rDNA of NA and molecular was identified by testing and comparing partal 16 S rDNA sequence of NA which further certificated the result of regular identification and biolog identification.
出处
《新疆农业大学学报》
CAS
2008年第3期39-42,共4页
Journal of Xinjiang Agricultural University
基金
国家自然科学基金项目(30471392)
高等学校博士学科点专项科研基金项目(20040298010)
关键词
松枯梢病
生防细菌
分子分类鉴定
16
S
RRNA
Sphaeropsis sapinea
antagonistic bacteria
molecular Identification
16 S rDNA
