尖锐湿疣皮损中β结合蛋白-1 mRNA的表达研究

Expression of latent TGF-β binding protein-1 mRNA in human condyloma acuminatum tissues

目的检测尖锐湿疣(CA)组织中潜在转化生长因子β结合蛋白-1(LTBP-1)mRNA表达水平,探讨其表达水平与CA发病的关系。方法用哺乳动物RNA抽提方法,提取CA组织总RNA,18s rRNA作为内参照,正常包皮组织作为对照,RT-PCR半定量分析LTBP-1 mRNA表达水平。结果CA组LTBP-1 mRNA表达水平比包皮组低,二者差异有统计学意义(P<0.01)。结论CA组织中LTBP-1表达下降,影响TGFβ功能的正常发挥,可能与CA疣体的发生有关。

Objective To detect the level of LTBP - 1 mRNA in condyloma acuminatum （CA） tissues amd explore the relationship between the expression of LTBP - 1 and the pathogenisis of CA, Methods Total RNA of CA tissues was extreacted , 18s rRNA as internal reference and normal foreskin as control The ezpression levell of LTBP - 1 mRNA was semiquantitatively analyzed. Results The levels of LTBP - 1 mRNA in CA group was lower than that of control group ,showing statistical significance （ P 〈 0.01 ）. Conclusion The expression level of LTBP - 1 is significantly lower in CA tissues. The decreased level of the cytokine in CA tissues is likely to interfere with the nomal function of TGF - β and it is probably associated with the pathogenisis of CA diseases.