萃取—紫外法测定乳香挥发油脂质体的包封率

Determination of entrapment efficiency of Boswellia carterii Birdwood volatile oil lyposomes by extraction–UV

目的建立测定乳香挥发油（BCBV）脂质体包封率的方法。方法采用石油醚萃取-紫外法测定乳香挥发油脂质体的包封率。结果乳香挥发油在307nm附近有最大吸收峰，浓度在41．36～310．20μg·ml^-1范围内与吸光度A呈良好的线性关系，精密度良好（RSD=0．3％），低、中、高三个浓度的乳香挥发油溶液平均回收率均符合规定，分别为98．5％、99．3％、98．1％。结论各项方法学考察指标均符合规定，适用于乳香挥发油脂质体包封率的测定。

Objective To establish a method for determination of the entrapment efficiency ofBoswellia carterii Birdwood volatile oil （to be abbreviated ： BCBV） liposomes. Methods Extraction - UV was adopted to measure the entrapment efficiency of BCBV liposomes. Results BCBV had the highest peak at 307nm in UV; a linear relationship was observed in the concentration rage of 41.36 -310.20μg·ml^-1; the precision was good（ RSD =0.3% ） ; the average recoveries of low ,middle and high concentrations were 98.5 %, 99.3% and 98.1%, respectively. Conclusion This method meets the requirements of methodologies and it is suitable for determination of the entrapment efficiency of BCBV hposomes.