莪术、哈蟆油对去势后大鼠雌激素水平及受体表达的影响

Effects of the mixture of Rhizoma Curcumae and Oviducts Ranae on estrogen and its receptor expressions in ovariectomized rats

目的检测莪术、哈蟆油对去势后大鼠血清雌激素水平、脾脏雌激素受体表达,探讨其对生殖内分泌-免疫网络的影响及作用机制。方法将成年SD雌性大鼠去势后制成更年期综合征模型,给药12周后,采用放射免疫法测定大鼠血清性激素(E2、FSH、LH、T、P)和白介素-2含量。取脾脏用免疫荧光法检测雌激素受体表达。结果与模型对照组比较大剂量莪术、哈蟆油能升高去势大鼠血清E2、T、P水平(P<0.01),明显提高IL-2含量(P<0.01),有降低FSH、LH含量趋势(P>0.05),并能显著升高脾脏ER表达水平(P<0.01)。结论莪术、哈蟆油可提高去势大鼠血清性激素水平,增强脾脏ER表达,为临床治疗更年期综合征提供实验依据。

Objective To study the effect of the mixture ofRhizoma Curcumae （YFN） and Oviducts Ranae on serum estrogen and spleen estrogen receptor expressions and its effect on reproductive endocrine-immune network in ovariectomized （OVX） rats. Methods Female SD rat models of climacteric syndrome （CS） were established by ovariectomy. After administration of YFN in the rats for 12 consecutive weeks, the serum estrogen levels （E2, FSH, LH, T, and P） and interleukin-2 were determined using radioimmunoassay, and the expression levels of estrogen receptor in the spleen were detected with immunofluorescence assay. Results The serum E2, T, P, and interleukin-2 levels （P〈0.01） and spleen estrogen receptor expression （P〈0.05） were significantly increased after high-dose YFN administration in the OVX rats as compared with the levels in the untreated OVX rats. YFN also resulted in lowered serum FSH and LH levels, but the changes were not statistically Significant. Conclusion YFN can increase the serum estrogen levels and estrogen receptor expression in the spleen of OVX rats, suggesting its clinical potential for relieving CS.