通下法对出口梗阻性便秘大鼠相关胃肠激素的影响

Effect of Catharsis on Levels of Plasma Motilin and Vasoactive Intestinal Polypeptide of Rats with Constipation by Outlet Obstruction

目的研究通下法对出口梗阻性便秘（OOC）大鼠血浆胃动素（motilin，MTL）、血管活性肠肽（vasoactive intestinal pepfide，VIP）的影响，探讨攻下法、润下法治疗OOC的合理性。方法采用直肠不全结扎法建立OOC动物模型，通过体外拆线解除梗阻，以大承气汤和麻仁丸为载体，观察通下法对大鼠血浆MTL和VIP的影响，以及结肠病理组织学改变。结果大承气汤组和麻仁丸组结肠黏膜炎症反应显著减轻，血浆MTL含量较正常组显著升高（P〈0．05）。模型组、大承气汤组和麻仁丸组大鼠血浆VIP的含量均显著低于正常组（P〈0．01）。结论攻下法和润下法均能减轻OOC大鼠肠黏膜炎症反应，影响血浆MTL、VIP含量，促进排便，但在临床应斟酌使用。

Objective To study the effect of catharsis method on the levels of plasma MTL and VIP of OOC rats, and investigate the rationality of purgation and lubricant purgation treating OOC. Methods The models of constipation by outlet obstruction were made by constricting the rectum of rats partly, and taking out stitches in vitro to relieve obstruction. Using the Da Cheng Qi Tang （DCQT） and Ma Ren Wan （MRW） as carderes to object the effect of catharsis method on the levels of plasma MTL and VIP of rats and contrast histopathologic severity of colitis. Results The inflammatory reaction of intestinal mucous was relieved significantly in DCQT group and MRW group, and levels of plasma MTL were raised significantly than normal group （P〈0.05）. The levels of plasma VIP went down significantly in model group, DCQT group and MRW group than in normal group（P〈0.01）. Conclusion Purgation and lubricant purgation could relieve the inflammatory reaction of intestinal mucosa of OOC rats, influence the levels of plasm MTL and VIE and promote defecation. But we must consider using them exactly.