外源基因转染对大肠癌细胞及放射敏感性的影响

The depressant effect of p16 gene transfection on colorectal cancer cell lines and its influence on radiotherapy sensitivity

目的探讨p16基因转染对大肠癌细胞生长及对放射敏感性的作用。方法以脂质体介导外源性P16基因转染大肠癌SWWC细胞系,照射后MTT法测定OD值,计算细胞生长抑制率。结果p16基因转染大肠癌SWWC细胞发生周期阻滞,照射后转染组细胞生长抑制率增加,与对照组有明显差异。结论p16基因转染对大肠癌SWWC细胞具有明显抑制作用,可提高大肠癌细胞对放射的敏感性。

Objective To observe the effects of pl6gene trangection on the growth and radiotherapy sensitivity ofcolorectal cancer cell lines.Methods Wild-type p16 gene was transfected imo SWWC colorectal cancer cell lines by lipofectamine. Being imadi- ate, MTr assay was used to determine the OD and calculate c611 growth restraint rate Results Colorectal cancer cells occur cycle blockage. Being irradiate, cell growth restraint rate increased of transaction group, it has the obvious difference with the control group. Conclusion It has depressant effect for colorectal cancer cell after p16 gene was transfected,and increase radiotherapy sensitivity of the colorectal cancer cells.