摘要
用0.1％的草酸作为流动相，高效液相色谱法测定了果珍中维生素C的含量。维生素C的浓度c在0-300μg／mL范围内与色谱峰面积A呈正比关系，线性相关系数为0.9993。方法的回收率为96．2％-103．2％，测定结果的相对标准偏差为0．6％（n=8）。利用高效液相色谱法测定果珍中的维生素c的含量，具有试剂用量少、操作简单、结果准确等特点，采用弱酸性的草酸为流动相，避免了维生素c的分解。
The content of Vitamin C was determined by high performance liquid chromatography with O. 1% oxalic acid as flow phase. Vitamin C concentration was linear with chromatography peak area in the range of 0 -300μg/mL, and the realative coffieient was 0.9993. The recoveries of Vitamin C in the juice powder were 96.2% - 103.2%. The relative standard deviation was 0.6% （ n = 8）. The virtue of the determination method by high performance liquid chromatography was economization of reagent, easy operation and accuracy. The decomposability of Vitamin C was avoided with oxalic acid as weak acidic flow phase.
出处
《化学分析计量》
CAS
2008年第3期19-20,共2页
Chemical Analysis And Meterage
基金
辽宁省教育厅高校科研计划项目(20060153)
大连民族学院博士科研启动基金资助项目(20056106)
关键词
维生素C
果珍
高效液相色谱
Vitamin C, juice powder, high performance liquid chromatography
