柯萨奇B组病毒3种检测方法的比较

Comparison of three methods for detecting coxsackie B group virus

目的探讨限制性内切酶分析法检测柯萨奇B组病毒的特异性和重现性。方法收集500例疑似病毒性心肌炎的患者血清,分别采用限制性内切酶分析法、ELISA法、RT-PCR法3种检测方法进行检测,每种方法在相同条件下连续重复3次,然后将其结果进行比较。结果ELISA法的3次检测的结果差别较大,阳性率分别为55.4%、58.4%、60.6%;RT-PCR法的3次检测的结果差别相对较小,阳性率分别为51.1%、51.6%、52.8%;限制性内切酶分析法的3次检测结果完全一致。结论限制性内切酶分析法是一种特异性强、重现性好,明显优于ELISA法和RT-PCR法的新的病毒检测方法。

Objective Study specificity and reproduction quality of restriction enzyme analysis method of detecting coxsackie B group virus. Method To gather 500 blood serum samples of patients （ diagnosis undetermined, DU）, apply restriction enzyme analysis, Elisa, RT - PCR analysis to detect samples three times, and contrast the results of each method. Results The detection results of samples by Elisa three times were diverse so far （55.4%, 58.4%, 60.6% ）; the detection results analyzed by RT - PCR have a little difference than former（51.1%, 51.6%, 52.8% ） ; the detection results of restriction enzyme analysis have excellent uniformity. Conclusion Restriction enzyme analysis which detected coxsackie B group virus was a high specificity and reproduction quality method compared with Elias and RT - PCR, this method showed a significant dominance.