HPLC同时测定冠通注射液中总绿原酸及灯盏花乙素含量

Simultaneous determination of Total chlorogenic acid and scutellarin in guantong injection by HPLC

目的建立冠通注射液含量测定方法。方法采用HPLC法，色谱柱为Diamonsil C18（250mm×4．6mm，5μm）；流动相为乙腈-0．4％磷酸溶液，线性梯度洗脱。采用二极管阵列检测器，选取与绿原酸相同紫外吸收图谱的峰为总绿原酸峰，在327nm检测波长处测定总绿原酸、灯盏花乙素含量。结果绿原酸在进样量0．333μg～8．880μg范围内呈良好的线性关系，回收率99．67％（RSI）=1．39％）；灯盏花乙素在进样量0．0804μg～1．34μg范围内呈良好的线性关系，回收率100．89%（RSD=0．72％）。结论方法准确可行，可用于该制剂的质量控制。

Objective To determine total chlorogenic acid and Scuteilarin in Guantong Injection Simultaneously by high-performance liquid chromatography （HPLC）. Methods A gradient elution method was set up with eluent of acetonitrile-water （0.4% phosphric acid） on a C18 reversed-phase column. Photodiode-array ultraviolet detector was used for the sake of choosing analogical peaks according to their ultraviolet aspects. The quantitative detection wavelength was set at 327 nm. Results The method presents good linearities in the range of 0.333 μ g～8.880 μ g and 0.0804 μ g～1.34 μ g for chlorogenic acid and scutellarin respectively and recoveries of 99.67% and 100.89% accordingly. Conclusion The method appears to he an adequate method for quality control in Guantong Injection.