胶原性关节炎大鼠滑膜组织G蛋白偶联受体激酶的表达及白芍总苷对其的影响

Expression of GRKs in synovial tissue from rats with collagen-induced arthritis and the effect of total glucosides of paeony

目的研究胶原性关节炎(CIA)大鼠滑膜组织中G蛋白偶联受体激酶(GRKs)表达以及白芍总苷(TGP)对其的影响。方法SD大鼠随机分成6组:正常组、模型组、TGP(25、50、100mg.kg-1)组、雷公藤(GTW40mg.kg-1)组。用鸡Ⅱ型胶原建立CIA大鼠模型,Western blot检测滑膜组织GRKs表达水平,并观察TGP灌胃给药对其的影响。结果正常SD大鼠滑膜组织GRK2、GRK5、GRK6均有表达,CIA大鼠关节滑膜组织GRK2、GRK5、GRK6的表达明显升高,TGP给药后GRK2,GRK5,GRK6的表达水平降低,差异有显著性。结论CIA滑膜组织GRKs表达增加,TGP抑制这种异常的变化可能是其治疗CIA的机制之一。

Aim To investigate the expression of GRKs in rats of synovial tissue with collagen-induced arthritis （CIA） and the effect of total glucosides of paeony. Methods SD rats were divided into six groups including normal, model, TGP （25,50,100 mg · kg^-1 ） groups, GTW （40 mg · kg^- 1 ） group. Chicken type Ⅱ collagen was used to induce CIA in rats. The expression of GRKs was detected by Westemblot. Results The expression of GRK2 ;5,6 increased in model group than that in normal group. Compared with the model group, the expression of GRK2,5,6 decreased in TGP CIA, the expression In rats of synovial tissue with of GRKs was abnormal, and TGP could change the variation of GRKs which may be one of the mechanisms of TGP improvement CIA.