益气养阴清热液对高血糖肥胖大鼠胰岛素抵抗的影响

Effects of Yiqi Yangyin Qingre Oral Liquid,a TCM fomulation on insulin resistance in hyperglycemic fatty rats

目的观察益气养阴清热液(YYQ)对高血糖肥胖大鼠胰岛素抵抗的改善作用。方法采用高脂饲料加小剂量链脲佐菌素(STZ)诱导高血糖肥胖大鼠胰岛素抵抗,用YYQ治疗4wk。检测空腹血糖(FBG)、葡萄糖耐量(OGT)、血清胰岛素(INS)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)、游离脂肪酸(FFA)、红细胞膜胰岛素受体(INSR)、胰岛素钳夹实验下的葡萄糖输注率(GIR);测定肾周、附睾周围脂肪垫重量。同时测定YYQ对肝组织中IR-1、IRS-1mRNA表达的影响。结果YYQ治疗4wk后,大鼠血清中TC、TG、LDL-C、INS、FFA降低,OGT改善,而血清中HDL-C和GIR明显升高,INSR高、低亲和力受体数目均明显增多,肝组织中IR、IRS-1mRNA表达升高,各项指标均与模型组大鼠差异有显著性。结论YYQ对高血糖肥胖大鼠胰岛素抵抗有治疗作用,通过增加胰岛素受体数目而提高其胰岛素敏感性可能是其作用机制之一。

Aim To investigate the Yiqi Yangyin Qingre Oral Liquid（YYQ） on insulin resistance in hyperglycemic fatty rats. Methods The model of hyperglycemic fatty rats was induced by intravenous injection with a low dose of streptozotocin（STZ） and a fed high fat and high caloric diet. The YYQ was given via gavage for a period of 4 weeks. The fasting blood glucose （ FBG）, oral glucose tolerance （ OGT）, serum insulin （ INS）, triglyceride （ TG ）, total cholesterol （ TC ）, free fatty acid （FFA） levels, glucose infusion rate （GIR） by euglycemic hyperinsulinemic clamp technique as well as erythrocyte insulin receptors （INSR） of rats were tested when the experiment was finished. The weight of the epididymal fat pads and the perirenal fat pads were measured. The IR, IRS-lmRNA expressions in liver tissue were measured by RT-PCR technology. Results YYQ treatment decreased the levels of FBG, TC, TG, LDL-C, FFA and increased the level of HDL-C in hyperglycemic fatty rats. As compared with the model rats, glucose tolerance was improved and body weight was reduced in hyperglycemic fatty rats treated with YYQ. Moreover, YYQ treatment upregulated IR, IRS- 1 mRNA expressions in liver tissue and increased insu- lin receptor amounts on the erythrocytes surface as well as GIR in the hyperinsulinemic euglycemic clamp experiment. Conclusions YYQ ameliorated insulin resistance in hyperglycemic fatty rats, and its mechanism might be related to increment of INSR and stimulation of insulin pathway.