杠柳苷对BT-549细胞增殖的抑制作用与p16、p27表达关系的研究

The Relationship between Suppressive Effects of Cortex Periplocae on BT-549 Cells and Expression of p16 and p27

背景与目的:观察杠柳苷对人乳腺癌细胞株BT-549体外增殖抑制的作用,检测p16和p27的表达,探讨杠柳苷抑制乳腺癌细胞的可能作用机制。材料与方法:以不同浓度的杠柳苷(2.5、5.0、10.0μg/ml)干预BT-549细胞,并设空白对照组,干预24、48、72h后,分别用MTT比色法分析细胞增殖情况;流式细胞技术检测细胞凋亡与周期分布。半定量RT-PCR、流式细胞技术检测细胞p16、p27mRNA和蛋白表达水平。结果:与对照组相比较,杠柳苷各实验组BT-549细胞增殖水平较对照组明显下降(P<0.01),促进细胞凋亡率明显增加(P<0.01),实验组细胞发生G0/G1期阻滞,细胞的p16、p27表达水平显著增高(P<0.01)。结论:杠柳苷具有抑制BT-549细胞增殖的作用,其机制可能与促进p16和p27表达水平的增高有关。

BACKGROUND AND AIM： The suppressive effects of cortex periploeae on human breast cancer BT-549 cells and the expressions of p16 and p27 were analyzed to demonstrate the possible mechanisms. MATERIALS AND METHODS： The suppressive effects of Cortex periplocae on proliferation of BT-549 cells was analyzed with MTT method. The cell cycle and apoptosis rate of BT-549 cells treated with Cortex periplocae were examined by flow cytometry. Expressions of p16 and p27 mRNA and proteins were assessed by semiquauntitative RT-PCR and flow cytometry. RESETS： Cortex periplocae could obviously inhibit proliferation of BT-549 cells （P 〈0.01）. Compared to control group, after treatment with Cortex periplocae, the cell number in Go/ G1 phase of BT-549 cells was increased（P 〈0.01）, the apoptosis rate was also increased significantly（P〈0.01） as well as the expression of p16 and p27（P〈0.05） CONCLUSION： Cortex periplocae exerted significant inhibitory effects on BT-549 cells in vitro which was probably related to improving the expressions of p16 and p27.