高效液相色谱法测定红果小檗中小檗碱的含量

Determination of Berberine Hydrochloride in Berberis Nummularia Bge.By HPLC

文章通过高效液相色谱法,建立红果小檗中小檗碱的含量测定方法。采用十八烷基硅烷键合硅胶为填充剂,迪马Diamonsil-C18色谱柱（4.6mm×250mm,5μm）;流动相为乙腈-0.1%磷酸（50∶50,每100mL加十二烷基磺酸钠0.1g）;检测波长：265nm;柱温：室温;流速：1.0 mL/min;进样量：10μL。线性关系：Y=4009.20X＋53.735,R=0.9998,线性范围0.027-1.354μg;回收率：平均回收率为99.43%,RSD=1.12%（n=6）。本法操作简便准确,具有分离度和重现性好、灵敏度高的特点,可作为该药材中小檗碱的含量测定和质量监控方法。

Objective：To establish a HPLC method for the determination of Berberine hydrochloride in Berberis nummularia Bge, Methods： A Diamonsil C18 column （4.6 mm×25 mm, 5μm） was used with the mobile phase of acetonitrile -0. 1 % phosphonic acid （50 ： 50, 0. 1g sodium dodecylsulphate/100 mL）. The detective wavelength was at 265nm. Results. The linear range of berberine hydrochloride was 0. 027-1. 354μg, Y = 4009.20X＋ 53. 735,R = 0. 9998, with the average recovery 99.43% , RSD=1. 12% （n = 6）. Conclusion： The method is simple and accurate with the features of good separation, sensation and reproduction. It may be used in the quality control and the determination of the content s of Berberine hydrochloride of this Chinese medicine meterial.