灯盏花素注射液对视网膜缺血再灌注损伤的保护作用

Protective effect of dengzhanhuasu against retinal ischemial reperfusion Injury

目的灯盏花素注射液对实验性视网膜缺血再灌注(retinal ischemial reperfusion injury,RIRI)的治疗作用及其可能的作用机理。方法将健康新西兰兔63只随机分为模型组/阴性对照组、低剂量组、高剂量组,3组均采用前房穿刺加压灌注使眼压升高至110mmHg(1kPa=7.5mmHg),维持60min,制作兔实验性RIRI模型。造模后随即每天1次按体重从耳缘静脉注入灯盏花素注射液,分别造成低、高剂量组,模型组同一时间从耳缘静脉注入等量生理盐水。分别于再灌注1d、3d、7d处死动物,光学显微镜和电镜下观察第3天的组织学变化和超微结构,分光光度法测量视网膜组织的丙二醛(malondialde-hyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)含量。结果再灌注1d,模型组视网膜MDA含量逐渐增高9.27±0.10,3d达高峰值9.55±0.47,7d趋于下降9.50±0.17;低、高剂量组MDA含量均较模型组显著降低(P<0.01)。再灌注1d,模型组视网膜SOD和CAT活性显著减低,3d达最低,7d趋于升高,低、高剂量组SOD、CAT活性均高于模型组(P<0.01)。结论灯盏花素注射液对实验性RIRI有保护作用,其机制可能是减少细胞内Ca2+超载、清除自由基和增强抗氧化能力。

Objective To investigate the protective effect of dengzhanhuasu on retinal ischemia-reperfusion injury（RIRI） and its possible mechanisms. Methods Sixty-three rabbits were randomly divided into model group, low dosage group and high dosage group. RIRI. model was established by increasing intraocular pressure to 110 mmHg （ 1 kPa = 7.5 mmHg ） for 60 minutes. Dengzhanhuasu was injected to low dosage group and high dosage group, and the same dosage of salt sodium was injected to model group. The rabbits were killed at 1 day, 3 days, 7 days after ischemia. Histological structure was observed under light microscope and electron microscope. The concentration of malondialdehyde （MDA） ,superoxide dismutase （SOD） and eatalase（ CAT）in the retina were measured with spectrophotometer. Results At one day after RIRI, MDA level of model group increased 9.27±0.10,and reached its peak 9.55±0.47 at 3 days after ischemia,then decreased slowly 9.50±0.17 at 7 days after RIRI. MDA level of both low dosage group and high dosage group were significantly lower than that of model group （P 〈0.01 ） ; SOD and CAT activity of model group were significantly lowered at 1 day after RIRI,to the lowest at 3 days after ischemia,to increase at the 7 days after RIRI. SOD and CAT activity of both low dosage group and high dosage group were higher than those of model group （P 〈 0. 01 ）. Conclusion Dengzhanhuasu can decrease cellular calcium overload and free radicals that protect experimental RIRI effectly.