摘要
目的培养和鉴定大鼠胚胎视网膜中的神经干细胞。方法取孕龄16～19dSD大鼠胚胎鼠的视网膜组织,在干细胞选择性培养基中培养,用免疫组织化学法来检测Nestin和Chx-10的表达。结果SD大鼠胚胎鼠视网膜中的神经干细胞在体外悬浮生长,形成致密的球形细胞团,传代后能够生成新的细胞团,原代和传代细胞Nestin和Chx-10蛋白均表达阳性。结论SD大鼠胚胎视网膜内存在神经干细胞,并且可以进行体外培养和扩增。
Objective To identify and culture the neural stem cells from fetal rat retina. Methods The cells which were taken from the SD fetal rat retinas were cultured in the special medium to obtain the stem cells,and immunocytochemical method was used to identify the expression of Nestin and Chx-10. Results The primary cultured cells could form a condensing sphere-shape cell cluster and suspended in the culture medium,and the passages also could form.The expression of Nestin and Chx-10 were detected in both the primary and passage cultured cells. Conclusion The fetal rat retina has neural stem cells that could be cultured and proliferated in vitro.
出处
《眼科新进展》
CAS
2008年第6期429-431,共3页
Recent Advances in Ophthalmology
