磷酸化细胞外信号调节激酶在卵巢浆液性癌中的表达和意义

Expression of p -ERK in ovarian serous cancer tissue and its biological significance

目的研究卵巢浆液性癌组织中P—ERK的表达特点及与其发生发展的关系。方法采用免疫组织化学方法对56例卵巢浆液性肿瘤（其中良性卵巢浆液性肿瘤11例，交界性卵巢浆液性肿瘤12例，卵巢浆液性癌33例）和10例正常卵巢组织进行p—ERK蛋白检测。结果卵巢浆液性癌组织的过表达率为75．6％（25／33），交界性卵巢浆液性肿瘤的过表达率为41．7％（5／12），均明显高于正常卵巢组织和良性卵巢浆液性肿瘤，它们之间比较差异有统计学意义（P〈0．05）；卵巢浆液性癌组织过表达率高于交界性卵巢浆液性肿瘤（P〈0．05）；卵巢浆液性癌患者不同临床分期（Ⅰ／Ⅱ期与Ⅲ／Ⅳ期）、病理分级及有无腹水、有无淋巴结转移间比较差异有统计学意义（P〈0．05）。结论p—ERK与卵巢浆液性癌发生发展的生物学行为和预后可能有关，可作为重要的生物学标记物。

Objective To study the relationship between p - ERK protein expression and its biological behaviors in ovarian serous cancer. Methods The expression of p - ERK in 56 specimens from patients with ovarian serous tumor which included three groups：33 samples of ovarian serous cancers; 12 samples of borderline ovarian serous tumors and 11 samples of benign ovarian serous tumors and 12 samples of normal ovarian tissues were detected by immunohistochemical method of strept audin - biotin complex. Results There was significantly higher overexpressive rate of p - ERK in ovarian serous cancers （ 75.6% ） and borderline ovarian serous tumors（41.7% ） than that in benign ovarian serous tumors and normal ovarian tissues（ P 〈 0.05）, and a higher overexpressive rate of p - ERK in ovarian serous cancers than that in borderline ovarian serous tumors （ P 〈 0.05 ）. Overexpressive rates of p - ERK were found significant differences in different clinical stages （ Ⅰ to Ⅱ and Ⅲ to Ⅳ ）, different histological grades, with or without ascites and lymphatic metastasis （ P 〈 0. 05 ）. Conclusion p - ERK may be associated with the carcinogenesis, biological behaviors and prognosis of ovarian serous cancer. It may be used as important biological marker in ovarian serous cancer.