毛细管电泳--多光子激发荧光检测分析生物胺

Determination of Biogenic Amines by Capillary Electrophoresis Coupling with Continuous Wave-based Multiphoton Excited Fluorescence Detection

构建了毛细管电泳-连续光多光子激发荧光检测系统并应用于生物分析.对几种常见生物胺,如腐胺,尸胺,组胺,精胺,亚精胺和苯乙胺的分析结果表明,该系统具有分离效率高、质量检测灵敏度高和低消耗等特点,适于复杂体系如生物样品的测量.定量分析显示,生物胺的检测限在nM级,线性范围超过2个数量级,进一步将多光子激发荧光检测与传统单光子激发荧光检测结果对比,发现前者还具有改善分离选择性的优势.

Multiphoton excited fluorescence （MPEF） detection coupling to capillary electrophoresis （CE） has been proposed to analyze biogenic amines, such as putrescine, cadaverine, histamine, spermine, spermidine and phenylethylamine. The detection limit was found to be nM level,with linear range of over two orders of magnitude for quantitation. It showed that the constructed CE-MPEF method was well suited for complex systems like bio-sample, with advantages of high separation performance, high detection sensitivity and low cost. Further, MPEF exhibited a capability for improving separation resolution after comparing with conventional single photon laser induced fluorescence.