脑缺血再灌注后沙鼠脑组织HSP27的表达变化和血清HSP27抗体的改变

The changes of HSP27 in brain tissue and its antibody in serum following cerebral ischemic reperfusion in gerbils

目的研究沙鼠脑组织热休克蛋白27（HSP27）的表达及血清抗体含量在脑缺血再灌注后的变化。方法采用夹闭双侧颈总动脉的方法制作沙鼠脑缺血再灌注模型；60只沙鼠随机分为正常对照组、假手术组、缺血再灌注组、分别在缺血再灌注后（IR）6h、1、3、7d的各时间点分批处死动物，双抗夹心法（ELISA）检测血清抗体量，采用免疫组织化学方法检测各组沙鼠脑内HSP27的表达情况，采用SPSSll．5软件进行统计分析。结果沙鼠缺血再灌注后，脑组织石蜡切片HE染色光镜观察，随缺血时间增加，神经元变性、坏死加重，胶质细胞增生明显，呈现损伤加重倾向。免疫组化检测正常对照组HSP27的阳性细胞为1．52±0，23，假手术组沙鼠脑组织中为1．74±0．24，2组比较差异无统计学意义（P〉0．25），阳性细胞主要位于胶质细胞胞浆和神经元中。缺血10min分别再灌注6h、1、3、7d后脑组织中HSP27阳性细胞逐渐增加，在3d时最多（35．12±2．39），与正常对照组及假手术组比较，差异有统计学意义（P〈0．01）；双抗夹心法检测血清HSP27量：假手术组在在6h，l，3，7d后抗体量变化很小，与正常对照组比较差异无统计学意义（P〉0．20）；缺血后再灌注组在6H，lD，3D，7D后，抗体量先逐渐上升，然后慢慢下降。结论沙鼠脑组织HSP27在脑缺血再灌注后表达较正常组、假手术组表达升高，提示HSP27表达的增加可能对机体起一定的保护作用。沙鼠血清HSP27抗体量在在脑缺血再灌注后表达较正常组、假手术组表达也升高，表明HSP27血清抗体可能对机体有损伤作用。

Objective To study the expressions of heat shock protein 27 （HSP27） in brain tissue and the changes of antibody of HSP27 in serum after transient cerebral ischemic reperfusion in gerbils. Methods A cerebral ischemia-reperfusion model in gerbils was es- tablished by clamping both common carotids. Sixty gerbils were randomly divided into normal control group, sham operation group and ische- mia-reperfusion （IR） group. The expressions of HSP27 in brain tissue were detected by immunohistochemistry technique and the changes of antibody of HSP27 were detected by ELISA technique at the 6th hour, 1st day, 3rd and 7th day after IR. Experimental results were analyzed with the statistic soft package of Spssl 1.5. Results Experimental results revealed that HSP27 were expressed in neurons and gliacytes of the brain tissue in the 60 gerbils. There was mild expressions of HSP27 in neurons and gliacytes in normal control group and sham operation group, but it has no significant difference between the two groups （ P 〉 0. 05 ）. Compared with normal control group, the expressions of HSP27 in neurons and gliacytes of ischemia-reperfusion gerbils were evidently increased at the 6th hour, 1 st day, 3rd day and 7th day, especially at the 3rd day （ P 〈 0. 01 ）. Condusiolas Expressions of HSP27 in neurons and gliacytes of gerbils were increased markedly. This suggested that the increased expressions of HSP27 could protect the brain tissue from damage. The changes of antibody in serum of normal control group, sham operation group were evidently less than that in group IR, which suggest the HSP27 antibody may damage our body.