花秆绿竹试管快速繁殖

Micropropagation of Bambusa oldhami f.striata by tissue culture

花秆绿竹Bambusa oldhamif. striata是绿竹Bambusa oldhami的一个变型。以花秆绿竹的侧芽为材料,研究不同质量浓度6-苄基腺嘌呤(BA)及噻二唑苯基脲(TDZ)对花秆绿竹试管快繁的影响。结果表明:单独添加BA或TDZ,随其质量浓度增加,增殖系数逐渐上升,但伸长生长受到抑制,添加TDZ的效果显著优于BA;BA和TDZ相互作用时,以3.00 mg.L-1BA与0.10 mg.L-1TDZ结合时芽体增殖及伸长生长最佳,芽丛生长旺盛;培养过程中降低或去除细胞分裂素即可生根。

Bambusa oldhami f. striata is a forma of Bambusa oldhami, having good ornamental value. This aim is to establish a tissue culture system for its commercial propagation. The new tender lateral bud were taken as the explants, Murashige and Skoog （MS） medium ＋30 g ·-L^-1 sugar ＋ 2.5 g·-L^-1 Gelrite was the basic medium, and 6-Benzylaminopurine （BA） （0, 0. 30, 1.00, 3.00, 10. 00 mg·-L^-1） and thidiazuron （TDZ） （0, 0.01, 0. 10, 1.00 mg·-L^-1） were added respectively to determine suitable concentration for tissue culture. The results showed that the shoot proliferation rate gradually increased with the concentration increasing of BA or TDZ, the effect of TDZ was better than BA, but the height growth of shoot was suppressed. 3.00 mg·-L^-1 BA ＋0. 10 mg ·-L^-1 TDZ was the best combination for shoot proliferation and shoot height growth. Declining or removing the cytokine could promote rooting. [ Ch, 2 tab. 10 ref. ]