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DYS712在山西汉族人群中遗传多态性分布

Polymorphism at DYS712 of Han Chinese ethnicity in Shanxi province
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摘要 目的对DYS712基因座的基因多态性和法医学意义进行研究。方法山西汉族人群中随机筛选110例无关男性,抽取静脉血提取DNA,对其筛选的等位基因测序,构建等位基因分型标准物;按照ISFG(国际法医遗传学会)原则命名各等位基因。结果本研究首次选择了Y染色体基因座DYS712,为4核苷酸复杂重复STR。重复单位为:[AGAT]+[A-GAC]。在110例山西汉族无关男性个体血样中共检出了6个等位基因:20、21、22、23、24和25。基因频率分别为0.0273,0.1909,0.318 2,0.2636,0.1545,0.0455。基因多样性为0.7732,在法医学应用方面,其个人识别能力(DP)和非父排出率(PE)均为0.7732。结论新Y染色体基因座DYS712具有较高的遗传多态性,在法医学及人类遗传学方面具有较高应用价值。 Objective To investigate the gene polymorphism of a locus DYS712 and its significance in forensic medicine. Methods DNA was extracted from 110 venous blood samples of unrelated male Han Chinese ethnicity in Shanxi province.Each allele of Y-STR loci was sequenced and the allele ladders were constructed.Alleles of Y-STR loci were nominated according to recommendations of the International Society of Forensic Genetics. Results The Y-STR loci DYS712 had four complex repeat nucleotides,and the sequence was [AGAT] ＋ [AGAC].Six alleles had been found in 110 unrelated male venous blood samples.They were 20,21,22,23,24 and 25.Their frequencies were 0.027 3,0.190 9,0.318 2,0.263 6,0.154 5 and 0.045 5,respectively.The gene diversity（GD）was 0.773 2.If used in forensic science,both the power of discrimination（PD）and the power of exclusion（PE）were 0.773 2.Conclusion The DYS712 has a high level of genetic polymorphism and can be used in forensic science and human genetics research.
作者 吴晓兵 张更谦 郭大玮 郝旭宇
机构地区 山西医科大学法医物证教研室
出处 《山西医科大学学报》 CAS 2008年第5期402-404,共3页 Journal of Shanxi Medical University
基金 山西省自然科学基金资助项目(20051046)
关键词 Y染色体短串联重复序列 汉族人群 DYS712 Y chromosome short tandem repeat Chinese Han population DYS712
分类号 Q347 [生物学—遗传学]
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山西医科大学学报
2008年 第5期

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