子宫腺肌病异位内膜细胞原代培养及鉴定

Primary culture and identification of ectopic endometrial cells from patients with adenomyosis

目的探讨子宫腺肌病异位内膜细胞原代培养,为进一步研究子宫腺肌病发病机制提供体外细胞模型。方法采用酶解、筛网分离、贴壁法分离异位内膜细胞。采用免疫细胞化学法进行细胞鉴定。结果经此法分离培养异位细胞培养成功率可达75.0%,消化得到的异位细胞种类较多,不容易分离提纯,腺细胞占6.5%,间质细胞占13.3%,平滑肌细胞占80.2%。结论采用酶解、筛网分离、贴壁法可以获得子宫腺肌病的异位细胞,不能获得纯度较高异位腺细胞,子宫腺肌病异位腺细胞提纯需要进一步探讨。

Objective To explore a method for primary culture of ectopic endometrial cells from patients with adenomyosis,and to find out a cell model in vitro for pathogenesis of adenomyosis. Methods The ectopic endometrial cells were isolated and purified by enzymolysis,filtration,and pasted wall purification,and identified by immunocytochemistry. Results The achievement ratio of isolating and culturing ectopic endometrial cells was 75.0%.The ectopic endometrial cells included glandular epithelial cell（6.5%）,stromal cell（13.3%）,and smooth muscle cell（80.2%）. Conclusion The ectopic endometrial cells of adenomyosis can be obtained by enzymolysis,filtration and pasted wall purification,but the more purified ectopic glandular epithelial cells cannot be obtained.