癌-睾丸抗原SSX-3的HLA-A2/A3限制性CTL广谱表位的预测

Prediction of HLA-A2/A3-restricted broad spectrum CTL epitope derived from the cancer-testis antigen SSX-3

目的:预测基于癌-睾丸抗原SSX-3的HLA-A2/A3限制性CTL广谱表位。方法:采用MULTI-PRED在线预测系统分别针对HLA-A2超型和HLA-A3超型对SSX-3序列进行CTL表位预测,其中对两种超型分值均较高的表位被初步认定为广谱表位;然后,运用NetCTL1.2在线预测系统对初步认定的广谱表位进行蛋白酶体裂解和TAP转运效率预测进一步筛选。结果:共预测出9个SSX-3抗原的HLA-A2/A3限制性广谱表位。结论:MULTIPRED与NetCTL1.2在线预测系统相结合可以高效的预测广谱CTL表位;本报道为SSX-3的广谱CTL表位的实验研究提供了重要的理论依据。

Objective：To predict the HLA - A2/A3 - restricted broad spectrum CTL epitope derived from the cancer-testis antigen SSX-3. Methods： On- line prediction server, MULTIPRED, was used to predict the CTL epitopes derived from SSX - 3 for both HLA - A2 and HLA - A3 supertypes. The epitopes showed higher scores for both two supertypes were considered as the possible broad spectrum epitopes. Then, NetCTL 1.2 server was used to predict the proteasomal cleavages and TAP transport efficiency of the possible broad spectrum epitopes. Results： Nine HLA- A2/A3 -restricted epitopes were predicted from the antigen SSX -3. Conclusion： MULTIPRED combined with NetCTL 1.2 server could predict broad spectrum CTL epitopes with high efficiency. Our results provided important evidence for the experimental studies of broad spectrum CTL epitopes derived from SSX -3.