肺癌细胞表面CAR表达水平与腺病毒转基因治疗的可能性研究

CAR expression level on lung cancer cell surface and potential adenovirus mediated gene therapy

目的:研究肺癌细胞表面柯萨奇腺病毒受体(CAR)表达水平与基因治疗的可能性。方法:用特异性CAR72抗体通过免疫组织化学Envision法在检测CAR在肺癌组织细胞中的表达水平。结果:在32例鳞癌中有25例CAR高表达、5例小细胞肺癌全部高表达,相反,CAR在22例腺癌中仅6例高水平表达。在非恶性肺泡上皮中为阴性。结论:以腺病毒血清型5为载体的基因治疗适合于鳞癌和非小细胞肺癌,而不适合于大多数腺癌。

Objective： To investigate the gene expression level of the Coxsackievirus and adenovirus receptor （CAR） locating on lung cancer cell surface and the potential strategy of gene therapy. Methods： Specific CAR72 antibody was used to detect CAR expression level in lung cancer cells of archived paraffin samples with the Envision immunohistochemical staining method. Results： High CAR expression were observed in five cases of small cell lung cancer , 25 of 32 pulmonary squamous cell carcinoma cases and only six of 22 lung adenocarcinoma. No CAR expression was detected in non - malignant alveolar epithelial. Conclusion： Gene therapy using adenovirus sera type 5 as the vector is suitable for treatment of pulmonary squamous cell carcinoma and non - small cell lung cancer but not for pulmonary adenocarcinoma.