CXCR4在胃癌中的表达及其意义

The significance of CXCR4 expression in gastric cancer

目的:观察趋化因子受体CXCR4在胃癌中的表达及其与胃癌临床病理因素的关系。方法:选取中国医科大学第一临床学院肿瘤外科确诊的胃癌患者30例,收集胃癌及相应癌旁组织,采用RT-PCR技术研究趋化因子受体CXCR4在各种病理类型、不同演进阶段的表达情况、表达分布情况。结果:趋化因子受体CXCR4在有淋巴结转移组的表达指数(0.34±0.12)显著高于无转移组的表达指数(0.23±0.07)(P<0.05),依照UICC分期标准其pN1组(0.26±0.04)与pN2+pN3组(0.51±0.09)也有显著性差异(P<0.05)。而与大体分型、浸润程度、分化程度、生长方式在本实验中无明显关系。结论:趋化因子受体CX-CR4的表达与淋巴结转移正相关,检测其表达对于推测胃癌淋巴结转移发生与否和转移程度有一定意义。

Objective.To determine the expression of chemokine （ C - X - C motif） receptor 4 （CXCR4） in gastric cancer and its relationship to clinicopathological characteristics. Methods： All specimens were obtained from 30 patients with gastric carcinoma who underwent operation at the Department of Surgery （ The First Affiliated Hospital of China Medical University）. Expression of CXCR4 in gastric carcinoma and uninvolved normal tissue was measured by reverse transcription - polymerase chain reaction （ RT - PCR）. And then the relationship of CXCR4 and gastric carcinoma clinicopathological characteristics were analyzed. Results ：The expression index of CXCR4 in lymph node metastasis positive group （0.34 ± 0. 12） was significantly higher than that in no metastasis negative group （0.23 ± 0.07 ） （ P 〈 0.05 ） , and the expression indexes of CXCR4 were （ 0.26 ± 0.04 ） and （0.51 ± 0.09 ） in gastric carci- noma stage pN1 and pN2 ± pN3. （P 〈0.05 ）. The expression of CXCR4 in gastric carcinoma and uninvolved normal tissue was not of significant difference observed in the present study. We noted no significant correlation between CXCR4 gene expressions in gastric carcinoma and the Borrmann gross type or depth of invasion or the Ming classification of growth pattern or grade of differentiation. Conclusion： CXCR4 gene expression is correlated with lymphatic metastasis in gastric carcinoma, therefore, testing the CXCR4 expression level in gastric carcinoma could be helpful in determining lymph node metastasis.