Survivin在大肠癌中的表达及意义

Expression of survivin and its significance in Colorectal Carcinoma

目的:检测抗凋亡基因survivin在大肠癌组织中的表达情况及与大肠癌组织生物学特性的关系。方法:收集62例大肠癌组织及25例正常大肠组织,采用RT-PCR方法,检测大肠癌中survivin mRNA表达水平,与正常大肠组织进行比较分析,并用免疫组织化学方法检测survivin蛋白表达。结果:大肠癌组织中sur-vivin mRNA阳性表达率为58.1%(36/62),大肠癌组织survivin蛋白阳性表达率为56.4%(35/62),而正常大肠组织无1例表达,且survivin表达与病理分级、有无淋巴结转移等恶性生物学特性密切相关。结论:Sur-vivin的高表达与大肠癌具有密切相关性,提示survivin对于大肠癌的预后可能具有重要意义。

Objective：To detect the expression of survivin in colorectal carcinoma and elucidate its function in colorectal carcinoma . Methods ： A total of 62 colorectal carcinoma specimens and 25 normal colorectal tissues were obtained. RT - PCR was used to measure survivin mRNA levels; Immunohistochemistry method was used to test protein expression of survivin. Results ： The expression rate of survivin mRNA in tumor tissues （ 58.1% , 36/62 ） was significantly higher than that in normal tissues （0,0/25） . Survivin protein was detected in 35 of 62 （56.4 % ） colorectal carcinoma tissues. In contrast , no expression of survivin in normal colorectal tissues was detected. Conclusion ： Overexpression of survivin is common in colorectal carcinoma. Survivin may play an important role in colorectal carcinoma and is associated with prognosis.