β-葡聚糖对巨噬细胞增殖胞内游离钙及cAMP浓度的影响

Effect of β-glucan on proliferation,in tracellular calcium and cAMP levels of RAW264.7 macrophage cell line

目的：探讨β-葡聚糖对RAW264.7细胞增生、胞内游离钙及cAMP浓度的影响。方法：中性红比色法检测细胞生长,酚试剂法检测细胞蛋白质合成,动态检测β-葡聚糖对胞内游离钙和cAMP浓度的影响。结果：微量β-葡聚糖可促进细胞生长及蛋白质合成;β-葡聚糖作用后3-5 min使胞内游离钙浓度升高,作用1 min后使cAMP浓度升高,并保持较高水平。结论：胞内游离钙及cAMP是β-葡聚糖对RAW264.7细胞作用过程中重要的信使分子。

Objective： To explore the effect of β-glucan on proliferation, intracellular calcium and cAMP levels of RAW264.7 macrophage cell line. Methods： Cell growth and proliferation were detected by neutral red colorimetric assay, protein synthesis by Lowry＇s test, and the concentration of cytosolic calcium and cAMP were determined continuously. Results： Cell growth and protein synthesis were enhanced by β- glucan ; the concentration of cytosolic calcium was elevated 3 - 5 min after treatment with β-glucan cAMP level increased 1 rain after the treatment and maintained at high level. Conclusion： Cytosolic calcium and cAMP were important signal molecules in evaluating effect of β-glucan on RAW264.7 cell line.