摘要
目的:了解局灶性脑缺血再灌注后Nogo-A的动态变化,以及三七三醇皂苷(PTS)对其表达的影响。方法:建立局灶脑缺血再灌注模型,采用免疫组织化学技术结合Western免疫印迹技术、功能评分,检测72只缺血再灌注后不同时期(3 d,7 d)脑内Nogo-A蛋白的表达并评价功能恢复。结果:Western免疫印迹和免疫组织化学均显示:Nogo-A表达在缺血再灌注损伤后3 d时下降,7 d时上升。PTS组在3 d时的Nogo-A表达比模型组低(P>0.05)。PTS组7 d时Nogo-A表达较模型组低(P<0.05)。结论:PTS可使缺血再灌注大鼠的Nogo-A表达下降,这可能是其发挥脑保护作用的机理之一。
Objective： To investigate the expression of Nogo-A and the effect of PTS on it following focal ischemia - reperfusion in the rat brain. Methods： The model of 72 cases of acute middle cerebral ischemia-reperfusion injury in rats established. The expression of Nogo-A protein in different durations of reper-fusion （3d,7d） was determined by Western blotting and immunohistochemical approach, and the functional scoring was also evaluated. Results： Western blotting showed Nogo-A proein expression was decreased on day 3 and increased significantly on day 7. Immunohistochemical approach showed the same results, The number of Nogo-A - immunoreactive cells at 3 d after reperfusion in PTS group decrease compared with the placebo group （P〉0.05）.And the number at 7 d in PTS group decreased markedly and represent significant difference compared with the placebo group（P〈0.05）. Conclusion： The study implies PTS could down- regulate the expression of Nogo-A , and it might be one of protective mechanisms of PTS against ischemiareperfusion injury.
出处
《中医药导报》
2008年第5期11-14,共4页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
