HPLC法测定根痛平胶囊中芍药苷含量

Determination of Paeoniflorin std. in Gentongping Capsule by HPLC

目的：建立根病平胶囊中芍药苷的含量测定方法。方法：色谱柱为Diamonisil（TM钻石）C18柱（200mm×4．6mm，5μm），流动相为甲醇-36％醋酸-水（25：2：73）；检测波长230nm；流速：1．OmL／min。结果：芍药苷进样量在0．560-1．680μg时呈良好的线性关系（r=O．9998）；加样回收率为99．8％，RSD为0．73％。结论：本法操作简便，分离效果好。

Objective： A HPLC method for determination of Paeoniflorin std. in Gentongping Capsule was established. Methods： The chromatographic conditions were listed below： Dianonisil ODS C18 column, UV detector at 230nm of wavelength, a mobile phase of carbinol：36%acetum：water （ 25：2：73 ）, and 1.0mL/ min of flow rate. Result ： The calibration curves were linear for naringin within 0.560-1.680μg （r=0.9998）. The recovery was 99.8% （ RSD=0.73% ） .Conclusion ： This method is simple and the separate result is satisfactory.