槲皮素对人卵巢癌HO-8910细胞增殖与凋亡的影响

The Effect of Quercetin on Proliferation and Apoptosis of HO-8910 Ovarian Cancer Cell

目的探讨槲皮素对人卵巢癌HO-8910细胞增殖与凋亡的作用。方法MTT法测定细胞增殖情况;流式细胞仪分析细胞周期及细胞凋亡;流式细胞仪检测槲皮素对凋亡相关蛋白Bcl-2、p53表达的影响。结果槲皮素对HO-8910细胞有增殖抑制作用,呈时间、剂量依赖性;流式细胞术检测结果分析,实验组G1/G0期上升,S期和G2/M期下降,细胞凋亡率上升;流式细胞术结果显示,槲皮素使Bcl-2表达下调,而上调p53蛋白的表达。结论槲皮素在体外能抑制卵巢癌HO-8910细胞的生长,阻止细胞由G1/G0期向S期和G2/M期移行并诱导细胞凋亡,其诱导HO-8910细胞凋亡的机制可能与上调促调亡蛋白p53表达和下调凋亡抑制蛋白Bcl-2表达有关。

Objective To study the effect of quercetin on proliferation and apoptosis of HO -8910 ovarian cancer cell in vitro. Methods The proliferation of cells was detected by MTT method. Cell cycle and apoptotic percentage were analyzed by flow Cytometry （FCM）. The expression of p53 and Bel - 2 were analyzed by FCM. Results Quercetin could inhibit the proliferation of HO - 8910 cell as direct relation of concentrations and times. The flow cytometry showed that the proportion of G1 phase and the apoptotic rate were increased significantly in quercetin groups, the proportion of S phase and G2/M phase was reduced. In the FCM quercetin induced upregulation expression of p53 and downregulation expression of Bcl -2. Conclusions Quercetin could inhibit growth and induce apoptosis of ovarian cancer HO- 8910 cell. Cell cycle was controlled in G1/G0. Meanwhile, quercetin could induce upregulation expression of p53 and downregulation expression of Bcl - 2, while may be the mechanism of apoptosis of ovarian cancer by quereetin.