三种染色方式下四种核酸染料的灵敏度比较

Comparative Analysis of the Sensitivity of Four Nucleic Acid Dyes Staining DNA by Three Staining Methods

目的探讨补镁对高脂高糖膳食诱导大鼠肥胖模型形成的影响及作用机制。使用染胶法、染样品法及后染法比较溴化乙锭(EB)与3种新型核酸染料GoldViewnaII、GoldView及DNA Green染色DNA的灵敏度。方法在琼脂糖凝胶电泳中,分别在3种染色方式下使用4种核酸染料对凝胶中的DNA进行染色,观察并分析电泳结果。结果核酸染料比较结果:GoldViewnaII的灵敏度最高,DNA Green的灵敏度与EB相当,GoldView的灵敏度略低于EB。染色方式比较结果:使用染胶法的灵敏度最高,后染法次之,染样品法最低。结论三种新型核酸染料完全可以替代EB,用于琼脂糖凝胶电泳DNA染色。使用新型核酸染料建议采用染胶法染色DNA。

Objective To compare the sensitivity of ethidium bromide（EB）and three types of new nucleic acid dye：GoldViewna II,GoldView and DNA Green staining DNA by three staining methods：prestaining gel method,prestaining sample method and poststaining method.Methods Four nucleic acid dyes were used to stain DNA respectively by three staining menthods in agarose gel electrophoresis and the results were observed and analyzed.Results The comparative result of nucleic acid dye：the sensitivity of GoldViewna II is highest of all;the sensitivities of DNA Green and EB are in the same degree;the sensitivity of GoldView is less than EB.The comparative result of staining method：The sensitivity of staining DNA by prestaining gel method is highest of the three and poststaining method is higher than prestaining sample method.Conclusion These three types of new nucleic acid dye can substitute for EB as a general dye to stain DNA in agarose gel electrophoresis.It is recommended to use Prestaining gel method when using new type of nucleic acid dye.