摘要
目的探讨乙醇诱导大鼠脑损伤时小脑维生素D依赖性钙结合蛋白28K(CaBP)表达及山莨菪碱的保护作用。方法2月龄SD雄性大鼠分别腹腔注射生理盐水、乙醇、山莨菪碱+生理盐水、山莨菪碱+乙醇(均n=18),8d后行Morris水迷宫测试,免疫组化结合图像分析系统计数小脑CaBP阳性表达的蒲肯野细胞平均面积百分比和灰度值。结果乙醇组Morris水迷宫潜伏期较其他3组延长(P<0.05),游泳距离较生理盐水组和山莨菪碱+生理盐水组延长(P<0.05),与山莨菪碱+乙醇组有延长的趋势,但无显著性差异(P>0.05)。乙醇组小脑CaBP阳性表达的蒲肯野细胞计数、平均阳性细胞面积百分比和灰度值均少于其他3组(P<0.05),山莨菪碱+乙醇组阳性细胞数与生理盐水组和山莨菪碱+生理盐水组无显著性差异(P>0.05),但平均阳性细胞面积百分比和灰度值降低(P<0.05)。结论乙醇可使小脑蒲肯野细胞的CaBP的表达减少;山莨菪碱对此具有一定保护作用。
Objective To investigate the effect of anisodamine on calbindin-D28K （CaBP） expression in the ethanol-induced brain damage in rat cerebellum. Methods 2 months aged male Sprague-Dawley rats were injected intraperitoeally with ethanol, normal saline, saline＋anisodamine and ethanol＋anisodamine respectively for 8 d. They were evaluated with Morris water maze. The counts, average area and density of CaBP positive neurons in cerebellum were measured with immunohistochemical technique and image analytical system. Results The latency of Morris water maze was significantly longer in the ethanol group than in the others （P〈0.05）, while the distance was significantly longer in the ethanol group than in the saline group and saline＋anisodamine group （P〈0.05）. There is not significant difference between ethanol group and ethanol＋anisodamine group （P〉0.05）, but is seemed some longer. The counts, average area and density of CaBP positive Purkinje cell were all significantly less in ethanol group than in the others （P 〈0.05）. There was not significant difference among ethanol＋anisodamine group, saline group and saline＋anisodamine group （P〉 0.05） in the counts, but the average areas and density in ethanol＋anisodamine group were less than those in saline group and saline ＋anisodomine group （P〈0.05）. Conclusion The ethanol can reduce the CaBP expression in the Purkinje cells of the rats cerebellum. Anisodamine can protect the rats cerebellum from it.
出处
《中国康复理论与实践》
CSCD
2008年第5期435-436,F0003,共3页
Chinese Journal of Rehabilitation Theory and Practice
