酿酒酵母体内氮的代谢作用

The Uptake of Nitrogen and Ammonia Assimilation by Saccharomyces Cerevisiae

酿酒酵母(Saccharomyces cerevisiae)可以在多种氮源上生长,通过转运蛋白的作用实现对培养基中氮源的吸收,然后在体内发生氨的同化作用代谢。氨不仅作为一种氮源,它同样是连接降解和生物合成途径的一个中间代谢物。氨的同化作用的调控涉及到编码酶基因的表达活性水平。为此,总结了酿酒酵母体内氮的吸收与氨同化作用,并且介绍了利用氨的同化作用的代谢工程学的研究进展。

Saccharomyces cerevisiae is able to use a wide variety of nitrogen sources for growth. The first step is that most nitrogen sources is uptake by specific permeates. The nest step is the degradation of the nitrogen source via ammonia assimilation. Ammonia plays a central role as intermediate between degradative and biosynthetic pathways. The role of ammonia assimilation is at the level of enzyme activity, translation, and transcription. This review describes the mechanism of the uptake of nitrogen sources and ammonia assimilation, and also the research on the metabolic engineering of the ammonia assimilation.