人膀胱粘膜／粘膜下层组织病变致组织漫反射光谱的变化

Pathological Changes of Human Bladder Mucosa/Submucosa Tissues Induced Changes in Diffuse Reflection Spectra of Tissues in Vitro

研究了人正常膀胱和浅表性膀胱癌的粘膜/粘膜下层组织在300～900nm光谱范围的漫反射光谱特性及其差异,实验采用带积分球附件的分光光度计获取组织的漫反射光谱。结果表明,在300～900nm,正常膀胱的粘膜/粘膜下层对任一个波长的漫反射率都明显地较癌变的粘膜/粘膜下层对相应波长的要大。正常膀胱的粘膜/粘膜下层的漫反射光谱的峰分别在370nm、520nm、550nm和660nm,其峰值分别为52.4%、60.7%、56.1%和75.5%。而癌变的粘膜/粘膜下层的漫反射光谱的峰分别在320nm、520nm、550nm和660nm,其峰值分别为43.7%、33.4%、30.6%和70.2%。正常膀胱的粘膜/粘膜下层的漫反射光谱在370nm处有一个峰,而癌变的粘膜/粘膜下层的漫反射光谱在370nm处没有峰,320nm处有一个峰。而正常膀胱的粘膜/粘膜下层的漫反射光谱在320nm处没有峰。膀胱的粘膜/粘膜下层病变导致组织的漫反射光谱在520nm、550nm和660nm处的峰的峰值分别减小了45.0%、45.5%和7.02%。说明膀胱的粘膜/粘膜下层病变导致组织的组分和结构尤其是组织中的氧合血红蛋白和去氧血红蛋白的含量发生了明显的改变。

Diffuse reflection spectra and their differences of normal human bladder and superficial human bladder cancer mucosa/submucosa tissues were studied in the spectral range from 300 nm to 900 nm in vitro. Diffuse reflection spectra of tissues were measured with a spectrophotometer with an integrating sphere attachment. The results of measurement show that diffuse reflection of normal bladder mucosa/submucosa tissue at each wavelength is obviously higher than that of superficial bladder cancer mucosa/submucosa tissue at the corresponding wavelength. The peaks in diffuse reflection spectra for normal bladder mucosa/submucosa tissues were 52.4 % at 370 nm, 60.7 % at 520 nm, 56.1% at 550 nm and 75.5% at 660 nm, respectively, while the peaks in diffuse reflection spectra for superficial bladder cancer mucosa/submucosa tissues were 43.7% at 320 nm, 33.4% at 520 nm, 30.6% at 550 nm and 70.2% at 660 nm, respectively. There was a peak in diffuse reflection spectra for normal bladder mucosa/ submucosa tissues at 370 nm, and there was not a peak for superficial bladder cancer mucosa/submucosa tissues at 370 nm. There was a peak in diffuse reflection spectra for superficial bladder cancer mucosa/submucosa tissues at 320 nm, and there was not a peak for normal bladder mucosa/submucosa tissues at 320 nm. Pathological changes of bladder mucosa/submucosa tissues induce the decrease of the peak values in diffuse reflection spectra for tissues of 45.0% at 520 nm, 45.5% at 550 nm, and 7.02% at 660 nm. The results show that pathological changes of bladder mucosa/submucosa tissues induce changes in the component and structure of tissues, and especially quantitative changes in oxyhemoglobin and de-oxyhemoglobin of tissues obviously.