小麦红叶耳基因的SSR标记定位分析

Mapping of Red-Auricle Gene in Wheat Using SSR Markers

为明确小麦红叶耳基因的染色体位置，以小麦BS210（红叶耳）／O201（绿叶耳）的DH群体为材料，于2005～2006和2006～2007年度分别将120个DH株系及亲本在北京种植，并对该群体进行叶耳颜色的鉴定。遗传和方差分析表明，红叶耳由一对主效基因控制，同时受环境影响，并存在基因与环境互作效应；根据DH群体叶耳颜色分别构建红叶耳池和绿叶耳池，利用BSA法和SSR引物筛选与红叶耳基因连锁的标记，将红叶耳相关基因定位于3B染色体长臂上，QTL分析表明，红叶耳主效QTL位于WMC182和WMC54之间，贡献率为24．1％，与最近标记WMC182的距离为7．1cM，红叶耳基因来自母本BS210。本研究结果还表明，BSA与QTL方法相结合，有利于提高简单性状基因定位的效率和精确度。

Red-auricle is one of important agronomic characters in wheat. It is easily influenced by en- vironment. Studies on genetic mapping of red-auricle using molecular markers were few reported in wheat. Identification of red-auricle genes and their closely linked molecular markers is very important for developing wheat cultivars. One DH population with 120 lines derived from a cross between BS210 and O201 was sown with two replicates in Beijing. The color investigation of auricle was carried out before tasseling during 2005-2006 and 2006-2007 cropping seasons. With the analysis of variance, the result showed that red-auricle is controlled by one pair of major gene, and it is influenced by he- redity, environment and exists interaction between gene and environment. The major red-auricle gene was mapped to chromosome 3B using Simple Sequence Repeat （SSR） markers and bulked segregant analysis （BSA）. Partial linkage maps around red-auricle loci in chromosomes 3B was constructed using the 120 lines of the DH population. With quantitative trait location （QTL） analysis, the major red-au- ricle gene of wheat was detected between the marker WMC182 and WMC54 in the 3BL, accounting for 24.1% of phenotypic variance. The distance to the nearest marker WMC182 is 7.1 cM, and the red- auricle gene derived from female parents BS210. This study demonstrated the feasibility in gene map-ping using bulked segregant analysis coupled with quantitative trait location（QTL）for simple traits.Also the results of mapping and molecular markers on red-auricle gene would be useful to identify the traits for varieties of wheat.