摘要
采用固相微萃取(SPME)和加速溶剂萃取(ASE)两种前处理方法从青山绿水茶叶(也叫苦丁茶)中提取挥发性成分,用气相色谱-质谱法(GC-MS)定性。实验结果表明:两种前处理方法共检测出91种成分,SPME检出49种,ASE检出56种,共同组分14种。青山绿水茶叶的挥发性成分主要成分有β-月桂烯、3,3,5-三甲基-1,5-庚二烯、L-柠檬烯、α-罗勒烯、β-罗勒烯、β-蒎烯、2-甲基安息香醛和5-羟甲基糠醛等物质。这两种提取方法各具优势,共同应用可以起到取长补短的作用。
The volatile components of Qingshanlvshui Tea were extracted using solid phase micro-extraction （SPME） and accelerated solvent extraction （ASE）, and then were identified by gas chromatography-mass spectrometry （GC-MS）. It showed that ninety-one compounds were identified, including forty-nine by SPME, fifty-six by ASE, and fourteen by both of them. The main constituents were β-myrcene, 3,5,5-trimethyl-1,5-heptadiene, L-limonene, α-ocimene, β-ocimene, β-pinene, 2-methylbenzaldehyde, 5-（hydroxymethyl）-2-furfural. Both SPME and ASE have their advantages. SPME is excellent at simplicity, rapidity, solvent-free, high enrichment, low detection limit, environment friendly etc. ASE has characteristics of time and solvent saving, automation, simplicity, as well as high efficiency.
出处
《色谱》
CAS
CSCD
北大核心
2008年第3期301-305,共5页
Chinese Journal of Chromatography
关键词
固相微萃取
加速溶剂萃取
气相色谱-质谱法
挥发性成分
青山绿水茶叶
solid phase microextraction （SPME）
accelerated solvent extraction （ASE）
gas chromatography-mass spectrometry （GC-MS）
volatile components
Qingshanlvshui Tea
