“热研11号”黑籽雀稗植株再生体系的建立

Tissue culture and Plant Regeneration of Tropical Pasture Paspalum atratum

以热带牧草"热研11号"黑籽雀稗(Paspalum atratum cv.Reyan No.11)种子为材料,对其外植体植株的再生过程进行系统研究。结果表明,以MS无机盐+9.0mg/L维生素B1+9.5mg/L维生素B6+4.5mg/L尼克酸+1.0mg/L水解酪蛋白+30.0g/L蔗糖+8.0g/L琼脂为基本成分(MSM),附加植物激素类物质2.0mg/L2,4-D时,适合种子的愈伤组织形成,愈伤诱导率可达65%;继代培养基附加1.0mg/L2,4-D和0.1mg/LKT;分化的培养基附加6.0mg/L6-BA,分化率可达50%;生根培养基附加0.5mg/L激素类物质NAA,生根率100%。完成植株再生约需13周。

A systematic study of plant regeneration of tropical pasture Paspalum atratum has been conducted.The result showed modified MS medium（MSM） composed of MS sah＋9.0 mg/L vitamin B1＋9.5 mg/L vitamin B6＋4.5 mg/L nicotinic acid＋1.0 mg/L casein hydrolisate ＋30 g/L sucrose＋8 g/L agar as basic medium was suitable for inducing callus formation from Paspalum atratum seeds when 2.0 mg/L 2,4-D was added, and gave a frequency of callus induction of 65%. The subculture medium was added with 1.0 mg/L 2,4-D and 0.1 mg/L KT. The MSM for differentiation at the presence of 6.0 mg/L 6-BA produced a frequency of green plantlets differentiation of 50 %. The rooting frequency was upto 100 % on the MSM medium with the supplement of 0.5 mg/L NAA. It takes about 13 weeks for Paspalum atratum to regenerate a plant.