氨溴索对急性肺损伤大鼠的抗氧化作用

Effect of ambroxol on oxidative stress in rats with acute lung injury

目的:探讨急性肺损伤(ALI)大鼠模型超氧化物歧化酶(SOD)和丙二醛(MDA)的变化及氨溴索对ALI的保护作用。方法:将36只Wistar大鼠随机分成对照组、油酸模型组、氨溴索组3组。氨溴索组在注入油酸前30min腹腔内注入氨溴索50mg/kg。以上各组均在注油酸后4h采血测SOD,观察肺组织病理形态学变化,测定肺匀浆中MDA。结果:氨溴索组血清SOD含量2 586±279 KU/L与模型组血清SOD含量1 898±256 KU/L比较明显增高(P<0.01);氨溴索组肺匀浆MDA含量(5.9±0.6nmol/ml)与模型组肺匀浆MDA(8.7±0.9nmol/ml)比较明显降低(P<0.01)。病理组织学观察示氨溴索组肺组织病理改变较模型组减轻。结论:氨溴索对油酸致ALI大鼠模型有保护作用,这种保护作用可能与其抗氧化反应有关。

Objective：To explore the change of SOD or MDA and protective effects of ambroxol on acute lung injury （ALI） induced by oleicacid in rats. Methods：36 Wistar rats were randomly divided into 3 groups： normal control group, oleicacid model group, ambr- oxol treated group（ injected intraperitoneally with 2ml/kg ambroxol at 30 minutes before oleicacid injection）. The changes of SOD or MDA in blood and pathlolgichistology were observed. Results： Serum SOD levels in ambroxol treated group （2586 ~ 279KU/L） were markedly increased compared with oleicacid model group（ 1898±256KU/L）. Pulmonary tissue damages were diminished by pretreat- ment with ambroxol. Conclusion：It is suggested that ambroxol have protective effects on the acute hmg injury induced by oleicacid, which maybe correlated with the function that ambroxol can inhibite lipidperoxide.