改性聚丙烯酰胺固定化苯酚降解菌的研究

Research on phenol degradation bacterium by immobilized modified polyacrylamide

利用自制的改性聚丙烯酰胺为载体包埋苯酚降解菌,考查了该载体对细胞性能的影响,比较了4种固定化方法——改性聚丙烯酰胺法、聚丙烯酰胺法、海藻酸钙法和聚乙烯醇-海藻酸钙法包埋微生物细胞的优劣。实验结果表明,单体丙烯酰胺经改性后制得的改性聚丙烯酰胺对微生物细胞活性无影响。以其为载体固定苯酚降解菌,其细胞相对活性比聚丙烯酰胺法高出了42.4%;比海藻酸钙法高出了16.4%;比聚乙烯醇-海藻酸钙法高出了44.3%,表明改性聚丙烯酰胺包埋细胞更有利于细胞的增殖和活性恢复。重复应用实验表明,改性聚丙烯酰胺法得到的细胞凝胶,机械强度好,有弹性,可多次重复利用。改性聚丙烯酰胺作为细胞固定化载体其优点是交联速度快、聚合放热温度低、在侧链发生交联反应、抗水解能力强、无毒、凝胶寿命长。

Phenol degrading bacteria were embedded by modified polyacrylamide as a carrier, and the impact on cell＇ s performance of carrier was examined ;advantages and disadvantages of embedding microbial cells immobilized by following four methods were compared：modified polyacrylamide method,polyacrylamide method, calcium alginate method and polyvinyl alcohol-calcium alginate method. Experimental results indicate that the modified polyacrylamide which was made by acrylamide monomer has no effect on microbial cells activity. Phenol degrading bacteria were immobilized by modified polyacrylamide as carrier , its relative activation of cell is 42.4% higher than the polyacrylamide method ; 16.4% higher than the calcium alginate method ; 44.3% higher than the polyvinyl alcohol-calcium alginate method, and these indicate that modified polyacrylamide is helpful to cell proliferation and cellular activity restoration. Repeated application experiments show that the mechanical intensity of cell gel which was received by modified polyacrylamide method is good,flexible and can be used repeatedly. The advantages of the modified polyacrylamide as immobile cell carrier are that crosslinking rate is rapid; the temperature of polymerization heat evolution is low ; crosslinking reaction occurs in the side-chain ; carrier resists the ability of hydrolysis strongly and is nontoxic; the gel is long-life.