微波放电激活氚标记方法研究

STUDIES OF TRITIUM LABELLING METHOD UTILIZING MICROWAVE DISCHARGE ACTIVATION OF TRITIUM GAS

本文介绍了一种改进的流气式双谐振腔微波放电激活氚标记方法,所建立的微波氘标记装置中,使用特制的玻璃往复泵实现了氚气的强制循环,使用两台微波发生器分别驱动两个谐振腔,诱导氚等离子体的产生,以进行氚标记。 研究了在微波氚标记时溴代环磷酸腺苷的脱卤和脱氢睾丸酮的加成作用,以期利用它们来合成高比活度的氚标记化合物。讨论了引入钯催化剂对微波氚标记,及微波氚标记过程中脱卤和加成作用的影响。介绍了一个样品进行多次重复微波标记的技术,用这种改进的微波氚标记方法可以同时合成多种氚标记化合物。

An improved method of tritium labelling utilizing double cavity microwave discharge activation of tritium gas is developed. The tritium labelling reaction apparatus consists of a closed vacuum system in which tritium gas can be cycled by a glass reciprocating pump. Two microwave generators are used to power the Evenson cavity and the McCarrol cavity to induce the tritium plasma separately. The tritium labelling reaction of the labelled substrate in the reaction area is initiated by microwave discharge activation of tritium gas.Dehalogenation of 8-BrcAMP and addition of 1-dehydrotestosterone by tritium atoms under the microwave discharge activation conditions are studied for the synthesis of [8-3H] cAMP and [1 ,2-3H] testosterone with the high specific activities.The influence of a palladium catalyst on the microwave tritium labelling reaction and dehalogenation or addition of the starting materials are discussed.In order to increase the specific activities of the tritium labelled compounds, a technique of labelling one and the same sample by the repeated microwave labelling operation is introduced. Several tritium labelled compounds can be prepared simultaneously by this new method.