聚己内酯纳米纤维膜固定化溶藻菌对藻类和藻毒素的生物降解作用

Biological degradation of algae and microcystins by algicidal bacteria immobilized on nanofiber membrane of polycaprolactone

采用电纺法制备并表征了聚己内酯纳米纤维膜(NMP).将从太湖水体中分离到的假单胞菌属溶藻菌固定化在聚己内酯纳米纤维膜(NMP)、维纶(VNL)和阿科蔓(AQUA)等人工介质材料上,分析与比较不同人工介质固定化溶藻菌对太湖土著铜绿微囊藻和微囊藻毒素LR(MC-LR)的生物降解作用.实验结果显示:NMP吸水率、孔隙率、拉伸强度分别为(81.33±1.58)%、(78.68±4.85)%、(89.8±7.5)MPa.NMP固定化溶藻菌的数量为5.65×107/cm2;NMP固定化溶藻菌对藻细胞的48h溶藻率和对MC-LR的48h降解率分别为85.68%和60.16%;溶解藻细胞可重复利用次数达到4次.上述结果表明,NMP是一种良好的固定化溶藻菌的纳米人工介质,可以增强溶藻菌对铜绿微囊藻和MC-LR的生物降解作用.

Nanofiber membrane of polycaprolactone was prepared by electrospinning and then characterized. The algicidal bacteria of pseudomonas strain isolated from Taihu Lake were then immobilized on three kind of artificial media of NMP （nanofiber membrane polycaprolactone, AQUA （Aquamats） and VNL （ Vinlon）, respectively. The algae-lytic and microcystin-degradative effects of the immobilized algicidal bacteria were detected and evaluated. The results show that the ratio of water absorptivity, the porosity and the tensile strength of the NMP are （81.33 ± 1.58）%, （78.68 ±4.85 ） %, （89.8 ± 7.5 ） MPa, respectively. The bacteria concentration of 30 h after immobilization on NMP is 5.65 × 10^7/cm^2. The 48 h algicidal ratio and microcystin-degradative ratio of NMP immobilized algicidal bacteria are 85.68% ,60. 16% respectively. The utilization of the NMP immobilized algicidal bacteria can be repeated for up to 4 times. NMP is a suitable material for bacteria immobilization by which the effects of immobilized algicidal bacteria on biological degradation of algae and microcystin LR can be improved.